Characterizing Service Level Objectives for Cloud Services: Motivation of Short-Term Cache Allocation Performance Modeling

Service level objectives (SLOs) stipulate performance goals for cloud applications, microservices, and infrastructure. SLOs are widely used, in part, because system managers can tailor goals to their products, companies, and workloads. Systems research intended to support strong SLOs should target realistic performance goals used by system managers in the field. Evaluations conducted with uncommon SLO goals may not translate to real systems. Some textbooks discuss the structure of SLOs but (1) they only sketch SLO goals and (2) they use outdated examples. We mined real SLOs published on the web, extracted their goals and characterized them. Many web documents discuss SLOs loosely but few provide details and reflect real settings. Systematic literature review (SLR) prunes results and reduces bias by (1) modeling expected SLO structure and (2) detecting and removing outliers. We collected 75 SLOs where response time, query percentile and reporting period were specified. We used these SLOs to confirm and refute common perceptions. For example, we found few SLOs with response time guarantees below 10 ms for 90% or more queries. This reality bolsters perceptions that single digit SLOs face fundamental research challenges.This work was funded by NSF Grants 1749501 and 1350941.No embargoAcademic Major: Computer Science and EngineeringAcademic Major: Financ

Utilization of Indole Acetic Acid with <i>Leucadendron rubrum</i> and <i>Rhododendron pulchrum</i> for the Phytoremediation of Heavy Metals in the Artificial Soil Made of Municipal Sewage Sludge

The development of phytoremediation by garden plants is an effective way to deal with the dilemma of municipal sewage sludge disposal. In this study, two ornamental plants were used as phytoremediation plants to rehabilitate heavy-metal-contaminated municipal sewage sludge in field experiments, and the role of exogenous phytohormone IAA was also tested. Ornamental plants Loropetalum chinense var. rubrum (L. rubrum) and Rhododendron pulchrum (R. pulchrum) adapted well to the artificial soil made of municipal sewage sludge, and the concentrations of Cu, Zn, Pb, and Ni were decreased by 7.29, 261, 20.2, and 11.9 mg kg−1, respectively, in the soil planted with L. rubrum, and 7.60, 308, 50.1, and 17.7 mg kg−1, respectively, in the soil planted with R. pulchrum, accounted for 11–37% of the total amounts and reached significant levels (p L. rubrum root. As a result, three months after transplant, the phyto-extraction amounts in L. rubrum were 397, 10.9, and 1330 μg for Ni, Cd, and Pb, respectively, increased by 233% to 279%. The phyto-extraction amount in R. pulchrum were 1510, 250, and 237 μg for Zn, Pb, and Cu, respectively, increased by 143% to 193%. These results indicated a potential to remediate heavy metals of the two ornamental plants, especially L. rubrum. The results of correlation analysis implied that the interaction of heavy metals in the plant itself played an important role in the uptake of heavy metals. This seemed to explain why applying IAA in the experiment had little effect on plant growth and phytoremediation of heavy metals. This study provided a green and feasible idea for the proper disposal of municipal sewage sludge

Src inhibition rescues FUNDC1-mediated neuronal mitophagy in ischaemic stroke

Background Ischaemic stroke triggers neuronal mitophagy, while the involvement of mitophagy receptors in ischaemia/reperfusion (I/R) injury-induced neuronal mitophagy remain not fully elucidated. Here, we aimed to investigate the involvement of mitophagy receptor FUN14 domain-containing 1 (FUNDC1) and its modulation in neuronal mitophagy induced by I/R injury.Methods Wild-type and FUNDC1 knockout mice were generated to establish models of neuronal I/R injury, including transient middle cerebral artery occlusion (tMCAO) in vivo and oxygen glucose deprivation/reperfusion in vitro. Stroke outcomes of mice with two genotypes were assessed. Neuronal mitophagy was analysed both in vivo and in vitro. Activities of FUNDC1 and its regulator Src were evaluated. The impact of Src on FUNDC1-mediated mitophagy was assessed through administration of Src antagonist PP1.Results To our surprise, FUNDC1 knockout mice subjected to tMCAO showed stroke outcomes comparable to those of their wild-type littermates. Although neuronal mitophagy could be activated by I/R injury, FUNDC1 deletion did not disrupt neuronal mitophagy. Transient activation of FUNDC1, represented by dephosphorylation of Tyr18, was detected in the early stages (within 3 hours) of neuronal I/R injury; however, phosphorylated Tyr18 reappeared and even surpassed baseline levels in later stages (after 6 hours), accompanied by a decrease in FUNDC1-light chain 3 interactions. Spontaneous inactivation of FUNDC1 was associated with Src activation, represented by phosphorylation of Tyr416, which changed in parallel with the level of phosphorylated FUNDC1 (Tyr18) during neuronal I/R injury. Finally, FUNDC1-mediated mitophagy in neurons under I/R conditions can be rescued by pharmacological inhibition of Src.Conclusions FUNDC1 is inactivated by Src during the later stage (after 6 hours) of neuronal I/R injury, and rescue of FUNDC1-mediated mitophagy may serve as a potential therapeutic strategy for treating ischaemic stroke

Distinct signatures of gut microbiota and metabolites in different types of diabetes: a population-based cross-sectional studyResearch in context

Summary: Background: Patients with type 1 diabetes (T1D) and type 2 diabetes (T2D) present intestinal disturbances. Recent epidemiological data have showed that, worldwide, over half of newly diagnosed T1D patients were adults. However, the gut microbial alterations in adult-onset T1D are unclear. We aimed to identify the signatures of gut microbiota and metabolites in patients with adult-onset T1D systematically, comparing with T2D patients and healthy controls (HCs). Methods: This study enrolled 218 subjects from February 2019 to April 2022 (discovery cohort: 36 HCs, 51 patients with adult-onset T1D and 56 patients with T2D; validation cohort: 28 HCs, 27 patients with adult-onset T1D and 20 patients with T2D). Gut microbial profiles of the study subjects were investigated by metagenomic sequencing, and their faecal and serum metabolites were measured with targeted metabolomics. The study was registered on ClinicalTrials.gov (NCT05252728). Findings: Patients with adult-onset T1D had significant differences in the composition of bacteria and their metabolites, characterized by notable depletion of short-chain fatty acid-producing bacteria, especially Eubacterium rectale. This was associated with a severe loss of phenolic acids and their derivatives, including gallic acid (associated with glucose metabolism) and 3,4-dihydroxyhydrocinnamic acid (linked with glucose metabolism and pancreatic beta cell autoimmunity). A predictive model based on six bacteria and six metabolites simultaneously discriminated adult-onset T1D from T2D and HCs with high accuracy. Interestingly, bacterial-viral or bacterial-fungal trans-kingdom relationships, especially positive correlations between bacteriophages and beneficial bacteria, were significantly reduced in adult-onset T1D compared to HCs. Interpretation: Adult-onset T1D patients exhibit unique changes in host-microbiota-metabolite interactions. Gut microbiota and metabolite-based algorithms could be used as additional tools for differential diagnosis of different types of diabetes and beyond. Funding: National Key Research and Development Program of China, the National Natural Science Foundation of China